Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUDT5 antibody [EPR7734] (ab129172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7734] to NUDT5
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NUDT5 antibody [EPR7734]
    See all NUDT5 primary antibodies

  • Description

    Rabbit monoclonal [EPR7734] to NUDT5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NUDT5 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: U937, HeLa, 293T and HepG2 cell lysates. IHC-P: Human kidney and Human bladder carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NUDT5 antibody [EPR7734] (ab129172)
    Western blot - Anti-NUDT5 antibody [EPR7734] (ab129172)
    All lanes : Anti-NUDT5 antibody [EPR7734] (ab129172) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NUDT5 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : U-937 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab129172 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab129172 Anti-NUDT5 antibody [EPR7734] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129172 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT5 antibody [EPR7734] (ab129172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT5 antibody [EPR7734] (ab129172)

    ab129172, at a 1/50, staining NUDT5 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-NUDT5 antibody [EPR7734] (ab129172)
    Western blot - Anti-NUDT5 antibody [EPR7734] (ab129172)
    All lanes : Anti-NUDT5 antibody [EPR7734] (ab129172) at 1/1000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT5 antibody [EPR7734] (ab129172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT5 antibody [EPR7734] (ab129172)

    ab129172, at 1/50, staining NUDT5 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-NUDT5 antibody [EPR7734] (ab129172)
    OI-RD Scanning - Anti-NUDT5 antibody [EPR7734] (ab129172)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NUDT5 antibody [EPR7734] (ab129172)
    Anti-NUDT5 antibody [EPR7734] (ab129172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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