Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FNTB antibody [EPR4706] (ab109748)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4706] to FNTB
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-FNTB antibody [EPR4706]
    See all FNTB primary antibodies

  • Description

    Rabbit monoclonal [EPR4706] to FNTB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: JAR, HepG2, K562, SH-SY5Y and C6 cell lysates. ICC/IF: K562 cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FNTB antibody [EPR4706] (ab109748)
    Western blot - Anti-FNTB antibody [EPR4706] (ab109748)
    Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified) + C6 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FNTB antibody [EPR4706] (ab109748)
    Western blot - Anti-FNTB antibody [EPR4706] (ab109748)
    All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified)

    Lane 1 : JAR whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : SH-SY5Y whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 49 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (ab109748)
    Immunocytochemistry/ Immunofluorescence - Anti-FNTB antibody [EPR4706] (ab109748)

    Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling FNTB with purified ab109748 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Western blot - Anti-FNTB antibody [EPR4706] (ab109748)
    Western blot - Anti-FNTB antibody [EPR4706] (ab109748)
    All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/1000 dilution (unpurified)

    Lane 1 : JAR cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 49 kDa

  • Anti-FNTB antibody [EPR4706] (ab109748)
    Anti-FNTB antibody [EPR4706] (ab109748)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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