Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Wednesday April 07, 2021

Anti-Histone H1.1 antibody [EPR23191-14] (ab254394)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23191-14] to Histone H1.1
Suitable for: Dot blot, ICC, Flow Cyt (Intra), WB, IHC-P
Reacts with: Human

Product name

Anti-Histone H1.1 antibody [EPR23191-14]
See all Histone H1.1 primary antibodies
Description

Rabbit monoclonal [EPR23191-14] to Histone H1.1
Host species

Rabbit
Tested applications

Suitable for: Dot blot, ICC, Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MDA-MB-468 whole cell lysate. IHC-P: Human testis and hepatocelluar carcinoma tissue. ICC: MDA-MB-468 cells. Flow Cyt: MDA-MB-468 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23191-14
Isotype

IgG

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.1 antibody (ab254394)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Histone H1.1 with ab254394 at 1/2000 (0.342 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human testis. The section was incubated with ab254394 overnight at 4°C. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Immunocytochemistry - Anti-Histone H1.1 antibody (ab254394)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-468 cells labelling Histone H1.1 with ab254394 at 1/1000 (0.683 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MDA-MB-468 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Histone H1.1 antibody [EPR23191-14] (ab254394)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-468 (Human breast adenocarcinoma epithelial cell) cells labelling Histone H1.1 with ab254394 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Histone H1.1 antibody (ab254394)

All lanes : Anti-Histone H1.1 antibody [EPR23191-14] (ab254394) at 1/1000 dilution

Lane 1 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 22 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: MDA-MB-231.

Exposure time: 3 minutes.
Dot Blot - Anti-Histone H1.1 antibody (ab254394)

Dot blot analysis of Histone H1.1 using ab254394 at 1/1000 (0.683 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate (ab97051) at 1/100,000 dilution.

Lane 1: Histone H1.1 peptide

Lane 2: Histone H1.2 peptide

Lane 3: Histone H1.3 peptide

Lane 4: Histone H1.4 peptide

Exposure time: 3 minutes.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.1 antibody (ab254394)

Immunohistochemical analysis of paraffin-embedded Human hepatocelluar carcinoma tissue labelling Histone H1.1 with ab254394 at 1/2000 (0.342 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on human hepatocelluar carcinoma. The section was incubated with ab254394 overnight at 4°C. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Anti-Histone H1.1 antibody (ab254394)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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