Anti-BAF170 antibody (ab264169)
Key features and details
- Rabbit polyclonal to BAF170
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-BAF170 antibody
See all BAF170 primary antibodies -
Description
Rabbit polyclonal to BAF170 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
-
Immunogen
Synthetic peptide within Human BAF170 aa 575-625. The exact sequence is proprietary. NP_003066.2
Database link: Q8TAQ2 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-BAF170 antibody (ab264169)All lanes : Anti-BAF170 antibody (ab264169) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 133 kDa
Exposure time: 3 minutes4-8% SDS-PAGE
Cells prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-BAF170 antibody (ab264169)BAF170 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264169 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264169 at 1 µg/ml.
Lane 1: ab264169 IP in HeLa whole cell lysate.
Lane 2: ab264169 IP in HeLa whole cell lysate.
Lane 3: Control IgG.Exposure time: 30 secs.
Different lots were used in lane 1 and lane 2.
Cells prepared using NETN lysis buffer.
