Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23066-21] to BAF170 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free
    See all BAF170 primary antibodies

  • Description

    Rabbit monoclonal [EPR23066-21] to BAF170 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, A549, HeLa, 293T, C2C12, Mouse brain, Mouse lung, Rat brain, Rat liver, C6, RAW264.7, PC-12 and NIH/3T3 lysates. IHC-P: Human stomach, Mouse hippocampus and Rat hippocampus tissues. ICC/IF: A549 and Neuro-2a cells. Flow Cyt: A549 and Neuro-2a cells. IP: K-562 cells.

  • General notes

    ab263871 is the carrier-free version of ab243634. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling BAF170 with ab243634 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in A549 cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

    PBS only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling BAF170 with ab243634 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human stomach. The section was incubated with ab243634 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling BAF170 with ab243634 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Neuro-2a cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

    PBS only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Flow Cytometry - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Flow Cytometry - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labelling BAF170 with ab243649 at 1/500  (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Immunoprecipitation - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Immunoprecipitation - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    BAF170 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab243634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243634 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10ug

    Lane 2: ab243634 IP in K-562 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243634 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling BAF170 with ab243634 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat hippocampus (PMID: 27392482). The section was incubated with ab243634 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling BAF170 with ab243634 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus (PMID: 27392482) The section was incubated with ab243634 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Flow Cytometry - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Flow Cytometry - Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (human lung carcinoma epithelial cell) cells labelling BAF170 with ab243634 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)
    Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free (ab263871)

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