Anti-C10orf46/CAC1 antibody [EPR16094] - BSA and Azide free (ab251041)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16094] to C10orf46/CAC1 - BSA and Azide free
- Suitable for: ICC, IP, WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-C10orf46/CAC1 antibody [EPR16094] - BSA and Azide free
See all C10orf46/CAC1 primary antibodies -
Description
Rabbit monoclonal [EPR16094] to C10orf46/CAC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251041 is the carrier-free version of ab190799 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251041 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as C10orf46
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR16094 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Human breast cancer lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDaThis data was developed using ab190799, the same antibody clone in a different buffer formulation.
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This data was developed using ab190799, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A375 cells labeling C10orf46/CAC1 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.
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This data was developed using ab190799, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling C10orf46/CAC1 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.
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This data was developed using ab190799, the same antibody clone in a different buffer formulation.Western blot analysis of C10orf46/CAC1 in immunoprecipitation pellets from HT1080 lysate. ab190799 used at 1/40 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46/CAC1 antibody [EPR16094] - BSA and Azide free (ab251041)This data was developed using ab190799, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling C10orf46/CAC1 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46/CAC1 antibody [EPR16094] - BSA and Azide free (ab251041)This data was developed using ab190799, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling C10orf46/CAC1 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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