Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
![Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)](https://www.abcam.com/ps/products/240/ab240051/Images/ab240051-403325-anti-hprt-antibody-epr5298-bsa-and-azide-free-western-blot.jpg "Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5298] to HPRT - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HPRT antibody [EPR5298] - BSA and Azide free
See all HPRT primary antibodies -
Description
Rabbit monoclonal [EPR5298] to HPRT - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab240051 is the carrier-free version of ab133242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240051 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5298 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
This data was developed using ab133242, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133242 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133242 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were used. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab133242 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/10,000 respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)](https://www.abcam.com/ps/products/240/ab240051/Images/ab240051-399134-anti-hprt-antibody-epr5298-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)All lanes : Anti-HPRT antibody [EPR5298] (ab133242) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDaThis data was developed using ab133242, the same antibody clone in a different buffer formulation.
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![Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)](https://www.abcam.com/ps/products/240/ab240051/Images/ab240051-3-benefits-of-recombinant-antibodies.png)
Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
