Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5298] to HPRT - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HPRT antibody [EPR5298] - BSA and Azide free
    See all HPRT primary antibodies

  • Description

    Rabbit monoclonal [EPR5298] to HPRT - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240051 is the carrier-free version of ab133242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240051 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
    Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)

    This data was developed using ab133242, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)

    Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: A431 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133242 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133242 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were used. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab133242 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/10,000 respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
    Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
    All lanes : Anti-HPRT antibody [EPR5298] (ab133242) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa



    This data was developed using ab133242, the same antibody clone in a different buffer formulation.

     

  • Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
    Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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