Anti-HPRT antibody [EPR5298] (ab133242)
![Anti-HPRT antibody [EPR5298] (ab133242)](https://www.abcam.com/ps/products/133/ab133242/Images/ab133242-270765-anti-hprt-antibody-epr5298-western-blot.jpg "Anti-HPRT antibody [EPR5298] (ab133242)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5298] to HPRT
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-HPRT antibody [EPR5298]
See all HPRT primary antibodies -
Description
Rabbit monoclonal [EPR5298] to HPRT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A synthetic peptide corresponding to residues in human HPRT (P00492).
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Positive control
- WB: Wild-type HAP1, HeLa, HepG2, A431 and MCF7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5298 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HPRT antibody [EPR5298] (ab133242)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab133242 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133242 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were used. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab133242 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/10,000 respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-HPRT antibody [EPR5298] (ab133242)](https://www.abcam.com/ps/products/133/ab133242/Images/ab133242-146648-anti-hprt-antibody-epr5298-western-blot.jpg)
Western blot - Anti-HPRT antibody [EPR5298] (ab133242)All lanes : Anti-HPRT antibody [EPR5298] (ab133242) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
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![Anti-HPRT antibody [EPR5298] (ab133242)](https://www.abcam.com/ps/products/133/ab133242/Images/ab133242-3-benefits-of-recombinant-antibodies.png)
Anti-HPRT antibody [EPR5298] (ab133242)
