Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7735] to NUDT5 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NUDT5 antibody [EPR7735] - BSA and Azide free
    See all NUDT5 primary antibodies

  • Description

    Rabbit monoclonal [EPR7735] to NUDT5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HeLa and HepG2 cell lysates.

  • General notes

    ab248321 is the carrier-free version of ab129163 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248321 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    Western blot - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab129163, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    This data was developed using ab129163, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    Western blot - Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NUDT5 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab129163).

    Lanes 1-4: Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)
    Anti-NUDT5 antibody [EPR7735] - BSA and Azide free (ab248321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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