Anti-HPRT antibody (ab233137)
Key features and details
- Rabbit polyclonal to HPRT
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-HPRT antibody
See all HPRT primary antibodies -
Description
Rabbit polyclonal to HPRT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey -
Immunogen
Recombinant full length protein corresponding to Human HPRT aa 1 to the C-terminus. N-terminal His-tag and GST-tag. Expressed in E.coli.
Database link: P00492 -
Positive control
- WB: Recombinant human HPRT protein; Rat liver and brain lysates; HeLa, HepG2 and A549 cell lysates. IHC-P: Human lung cancer, liver, liver cancer, glioma, breast cancer and prostate tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Images
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Anti-HPRT antibody (ab233137) at 5 µg/ml + Recombinant human HPRT protein
Predicted band size: 25 kDa
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All lanes : Anti-HPRT antibody (ab233137) at 3 µg/ml
Lane 1 : Rat liver lysate
Lane 2 : Rat brain lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : A549 (human lung carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 25 kDa
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for HPRT using ab233137 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for HPRT using ab233137 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver cancer tissue stained for HPRT using ab233137 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for HPRT using ab233137 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for HPRT using ab233137 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for HPRT using ab233137 at 10 μg/ml in immunohistochemical analysis. DAB staining.