Human TNFRSF14 Matched Antibody Pair Kit (ab220130)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Human TNFRSF14 Matched Antibody Pair Kit
See all TNFRSF14/HVEM kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.2 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human TNFRSF14 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human TNFRSF14.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216950. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human TNFRSF14 Capture Antibody 2 x 50µg 1 x 50µg Human TNFRSF14 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human TNFRSF14 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. -
Tissue specificity
Widely expressed, with the highest expression in lung, spleen and thymus. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- HVEML
- ATAR
- CD270
see all -
Database links
- Entrez Gene: 8764 Human
- Omim: 602746 Human
- SwissProt: Q92956 Human
- Unigene: 512898 Human
Images
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Human TNFRSF14 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human TNFRSF14 Matched Antibody Pair Kit (ab220130)To learn more about the advantages of recombinant antibodies see here.
