Inosine Quantification Assay Kit (Fluorometric) (ab126286)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 nmol/well
Overview
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Product name
Inosine Quantification Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
0.1 nmol/well -
Assay time
0h 40m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Inosine Quantification Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.
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Notes
Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Converter Enzyme Mix (Lyophilized) Green 1 vial Developer Enzyme Mix (Lyophilized) Brown 1 vial Inosine Assay Buffer WM 1 x 25ml Inosine Standard (10 mM) Yellow 1 vial Inosine Substrate Mix (Lyophilized) Red 1 vial PicoProbe Blue 1 x 0.4ml -
Research areas
Images
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Functional Studies - Inosine Assay Kit (ab126286)
Inosine standard curve generated using this kit protocol.