Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HINT1 antibody [EPR5108] (ab124912)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5108] to HINT1
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-HINT1 antibody [EPR5108]
    See all HINT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5108] to HINT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human HINT1. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, 293T, MCF7, HepG2 and Jurkat cell lysates. IHC-P: Human breast carcinoma and Human colonic adenocarcinoma tissues. ICC/IF: MCF7 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)
    Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)
    All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : HINT1 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab124912 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab124912 Anti-HINT1 antibody [EPR5108] was shown to specifically react with HINT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265776 (knockout cell lysate ab257465) was used.  Wild-type and HINT1 knockout samples were subjected to SDS-PAGE.  ab124912 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)
    Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)
    All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HINT1 antibody [EPR5108] (ab124912)
    Immunocytochemistry/ Immunofluorescence - Anti-HINT1 antibody [EPR5108] (ab124912)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling HINT1 with purified ab124912 at 1/1000 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)

    ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)

    ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-HINT1 antibody [EPR5108] (ab124912)
    Anti-HINT1 antibody [EPR5108] (ab124912)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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