Anti-HINT1 antibody (ab264398)
Key features and details
- Rabbit polyclonal to HINT1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HINT1 antibody
See all HINT1 primary antibodies -
Description
Rabbit polyclonal to HINT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
-
Immunogen
Synthetic peptide within Human HINT1 aa 1-50. The exact sequence is proprietary.
Database link: P49773 -
Positive control
- IP: HEK-293T whole cell lysate.
-
General notes
Anti-HINT1 antibody (ab264398) has not performed satisfactorily when used for WB of HINT1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HINT1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunoprecipitation - Anti-HINT1 antibody (ab264398)
HINT1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264398 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264398 at 1 µg/ml.
Lane 1: ab264398 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
