Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5108] to HINT1 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HINT1 antibody [EPR5108] - BSA and Azide free
    See all HINT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5108] to HINT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells WB: HeLa and Jurkat cell lysates. IHC-P: Human breast carcinoma and colonic adenocarcinoma tissues.

  • General notes

    Ab240007 is the carrier-free version of ab124912. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240007 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)
    Western blot - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)
    All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : HINT1 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab124912).

    Lanes 1-3: Merged signal (red and green). Green - ab124912 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab124912 Anti-HINT1 antibody [EPR5108] was shown to specifically react with HINT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265776 (knockout cell lysate ab257465) was used.  Wild-type and HINT1 knockout samples were subjected to SDS-PAGE.  ab124912 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

    ab124912, at 1/250 dilution, staining HINT1 in human paraffin embedded breast carcinoma tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124912).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)
    Immunocytochemistry/ Immunofluorescence - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling HINT1 with purified ab124912 at 1/1000 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124912).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

    ab124912, at 1/250 dilution, staining HINT1 in human paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124912).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)
    Anti-HINT1 antibody [EPR5108] - BSA and Azide free (ab240007)

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