HRP Anti-SF3a66 antibody [4G8] (ab201747)
![HRP Anti-SF3a66 antibody [4G8] (ab201747)](https://www.abcam.com/ps/products/201/ab201747/Images/ab201747-250650-IHCAP24094281.jpg "HRP Anti-SF3a66 antibody [4G8] (ab201747)")
Key features and details
- HRP Mouse monoclonal [4G8] to SF3a66
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-SF3a66 antibody [4G8]
See all SF3a66 primary antibodies -
Description
HRP Mouse monoclonal [4G8] to SF3a66 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Full length native protein (purified) corresponding to Rat SF3a66. Nuclear protein isolated from rat liver nuclei.
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Epitope
C-terminal, amino acids 212-464. -
Positive control
- IHC-P: FFPE human skin tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
4G8 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SF3a66 antibody [4G8] (ab201747)
IHC image of SF3a66 staining in a section of formalin-fixed paraffin-embedded human normal skin*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201747, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
