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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes Hect E3 Ligase

Anti-HACE1 antibody [EPR7962] (ab133637)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-HACE1 antibody [EPR7962] (ab133637)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7962] to HACE1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-HACE1 antibody [EPR7962]
    See all HACE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7962] to HACE1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human HACE1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T and SH-SY5Y cell lysates
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7962
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Hect E3 Ligase
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Images

  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain lysate at 20 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : SH-SY5Y whole cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    Western blot - Anti-HACE1 antibody [EPR7962] (ab133637)
    All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate at 10 µg

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 102 kDa

  • Anti-HACE1 antibody [EPR7962] (ab133637)
    Anti-HACE1 antibody [EPR7962] (ab133637)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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