Anti-HACE1 antibody [EPR7962] (ab133637)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7962] to HACE1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HACE1 antibody [EPR7962]
See all HACE1 primary antibodies -
Description
Rabbit monoclonal [EPR7962] to HACE1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide within Human HACE1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- 293T and SH-SY5Y cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7962 -
Isotype
IgG -
Research areas
Images
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Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)
Lane 1 : HEK293 whole cell lysate
Lane 2 : SH-SY5Y whole cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate at 10 µg
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
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