Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)
![Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)](https://www.abcam.com/ps/products/264/ab264431/Images/ab264431-355621-AntiSC35-antibody-SC35--BSA-and-Azide-free-immunocytochemistry-hela-human.jpg "Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)")
Key features and details
- Mouse monoclonal [SC-35] to SC35 - BSA and Azide free
- Suitable for: ELISA, IHC-P, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SC35 antibody [SC-35] - BSA and Azide free
See all SC35 primary antibodies -
Description
Mouse monoclonal [SC-35] to SC35 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Epitope
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. -
Positive control
- ICC/IF: HeLa cells.
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General notes
ab264431 is the PBS only version of ab11826.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
SC-35 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)
This image was produced using the same antibody clone but in a different formulation ab11826.
ICC/IF image of ab11826 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11826 at 1 µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
