Anti-SRPK1 antibody (ab90527)
Key features and details
- Rabbit polyclonal to SRPK1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SRPK1 antibody
See all SRPK1 primary antibodies -
Description
Rabbit polyclonal to SRPK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide within Human SRPK1 aa 275-325 (internal sequence). The exact sequence is proprietary. (GeneID 6732)
Database link: NP_003128.3 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling SRPK1 with ab90527 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-SRPK1 antibody (ab90527) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 10 seconds
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ab90527 at 1 µg/ml detecting SRPK1 following immunoprecipitation. Detection by ECL with a 3 second exposure.
Lane 1; IP with ab90527
Lane 2; IP with an antibody to a downstream epitope of SRPK1
Lane 3; IP with control IgG
All antibodies used at at 3 µg/mg lysate for immunoprecipitation. 1 mg of lysate was used for IP and 20% of IP loaded.