Anti-PARP1 antibody (ab194586)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Rabbit polyclonal to PARP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PARP1 aa 150-250. The exact sequence is proprietary.
Database link: P09874 -
Positive control
- WB: Extracts from A549, Jurkat, HeLa, K562, Raji, HepG2 and Y79 cell lines. Mouse testis lysate. ICC/IF: NIH/3T3 and U-2 OS cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PARP1 antibody (ab194586) at 1/1000 dilution
Lane 1 : Extract from A549 (Human lung carcinoma cell line) cell line
Lane 2 : Extract from Jurkat (Human T cell leukemia cell line from peripheral blood) cell line
Lane 3 : Extract from Hela (Human epithelial cell line from cervix adenocarcinoma) cell line
Lane 4 : Extract from K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell line
Lane 5 : Extract from Raji (Human Burkitt's lymphoma cell line) cell line
Lane 6 : Extract from HepG2 (Human liver hepatocellular carcinoma cell line) cell line
Lane 7 : Extract from Y79 (Human retinoblastoma cell line) cell line
Predicted band size: 113 kDa
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U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for PARP1 using ab194586 at a dilution of 1/100 in ICC/IF. Blue: DAPI for nuclear staining.
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Immunohistochemistry of Human breast cancer tissue staining PARP1 with ab194586
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Immunohistochemistry of Human appendix tissue staining PARP1 with ab194586
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Immunohistochemistry of rat testis tissue staining PARP1 with ab194586
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NIH-3T3 (Mouse embryo fibroblast cell line) cells stained for PARP1 using ab194586 at a dilution of 1/100 in ICC/IF. Blue: DAPI for nuclear staining.
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Anti-PARP1 antibody (ab194586) at 1/1000 dilution + Mouse testis lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 90 secondsBlocking buffer: 3% non-fat dry milk in TBST.