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Recombinant human PARP1 protein (Active) (ab271649)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 90% SDS-PAGE
  • Active: Yes
  • Tags: DDDDK tag N-Terminus, Avi tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PARP1 protein (Active)
    See all PARP1 proteins and peptides

  • Biological activity

    Enzyme reaction is conducted using a PARP Assay Kit at room temperature for 1 hour.

  • Purity

    >= 90 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    P09874

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      117 kDa

    • Amino acids

      1 to 1014

    • Tags

      DDDDK tag N-Terminus , Avi tag N-Terminus

Preparation and Storage

  • Alternative names

    • ADP ribosyltransferase
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
    • ADP ribosyltransferase diphtheria toxin like 1
    • ADP ribosyltransferase NAD(+)
    • ADPRT
    • ADPRT 1
    • ADPRT1
    • ARTD1
    • msPARP
    • NAD(+) ADP ribosyltransferase 1
    • NAD(+) ADP-ribosyltransferase 1
    • pADPRT 1
    • pADPRT-1
    • pADPRT1
    • PARP
    • PARP 1
    • PARP-1
    • PARP1
    • PARP1_HUMAN
    • Poly (ADP ribose) polymerase 1
    • poly (ADP ribose) polymerase family, member 1
    • Poly (ADP-ribose) polymerase 1
    • Poly [ADP-ribose] polymerase 1
    • Poly(ADP ribose) polymerase
    • poly(ADP ribose) synthetase
    • poly(ADP ribosyl)transferase
    • Poly(ADP-ribosyl)transferase
    • Poly[ADP ribose] synthetase 1
    • Poly[ADP-ribose] synthase 1
    • PPOL
    • sPARP 1
    • sPARP1
    see all

  • Function

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.

  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PARP1 protein (Active) (ab271649)
    Functional Studies - Recombinant human PARP1 protein (Active) (ab271649)
    Specific activity of ab271649.
  • SDS-PAGE - Recombinant human PARP1 protein (Active) (ab271649)
    SDS-PAGE - Recombinant human PARP1 protein (Active) (ab271649)

    SDS-PAGE analysis of 3.8 µg ab271649.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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