Astana Biomed Group, an authorized Abcam distributor in Central Asia

Stimulatory Immune Checkpoint panel 2 - Human WB (ab278177)

Overview

  • Product name

    Stimulatory Immune Checkpoint panel 2 - Human WB

  • Species reactivity

    Reacts with: Human

  • Product overview

    Stimulatory Immune Checkpoint panel 2 - Human WB ab278177 contains multiple trial-sized versions of anti-human antibody clones against CD28, CD27, CD226, TMEM173, GITR and TNFSF18/GITRL specifically selected for high performance in various applications.  They are provided as a sampler panel to allow you to easily evaluate each antibody.


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [EPR22076] to CD28 (20 μL) ab243228


    - Rabbit monoclonal [EPR8569] to CD27 (20 μL) ab131254


    - Rabbit monoclonal [EPR21172] to CD226 (20 μL) ab212076


    - Rabbit monoclonal [EPR13130-55] to TMEM173 (20 μL) ab239074


    - Rabbit monoclonal [EPR20566] to GITR (20 μL) ab223841


    - Rabbit monoclonal [EPR20583] to TNFSF18/GITRL (20 μL) ab211037

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Please refer to the ‘Associated products’ section below.

    Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Please refer to the ‘Associated products’ section below.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-TNFSF18/GITRL antibody [EPR20583] (ab211037) at 1/5000 dilution.

    Lane 1: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate, 10 µg

    Lane 2: THP-1 (Human monocytic leukemia cell line) whole cell lysate, 10 µg

    Secondary (all lanes): ab97051 at 1/100000 dilution.

    Predicted MW: 23 kDa.

    Observed MW: 20 kDa.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    TNFSF18 is constitutively expressed in HUVEC cells but is not expressed in monocytic cell line.

    Negative control: THP-1 (PMID: 10037686).

    Exposure: 3 minutes

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-GITR antibody [EPR20566] (ab223841) at 1/1000 dilution.

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a Myc-GFP-tag whole cell lysate, 10 µg

    Lane 2: HEK-293T transfected with human GITR, containing a Myc-GFP-tag, whole cell lysate, 10 µg

    Secondary (all lanes): ab97051 at 1/100000 dilution.

    Predicted MW: 26 kDa.

    Observed MW: 52 kDa.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure: 1 second

    ECL

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-TMEM173 antibody [EPR13130-55] (ab239074) at 1/1000 dilution.

    Lane 1: THP-1 (human monocytic leukemia cell line) whole cell lysate, 20 µg

    Lane 2: HT1080 (human fibrosarcoma cell line) whole cell lysate, 20 µg

    Lane 3: Human tonsil lysate, 20 µg

    Secondary (all lanes): ab97051 at 1/100000 dilution.

    Predicted MW: 42 kDa.

    Observed MW: 36 kDa.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure: 3 minutes

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-CD226 antibody [EPR21172] (ab212076) at 1/1000 dilution.

    Lane 1: HEL (human bone marrow erythroleukemia cell line) whole cell lysate, 20 µg

    Lane 2: Human fetal spleen lysate, 20 µg

    Lane 3:     Human thymus lysate, 20 µg

    Secondary (all lanes): ab97051 at 1/100000 dilution.

    Predicted MW: 39 kDa.

    Observed MW: 60-80 kDa.

    Exposure time : Lane 1: 37 seconds; Lane 2: 15 seconds; Lane 3: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The different molecular masses observed in different tissues or cell lines may be due to different level of CD226 glycosylation (PMID: 8673704).

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-CD27 antibody [EPR8569] (ab131254) unpurified, at 1/1000 dilution.

    Lane 1: Ramos (human Burkitt's lymphoma cell line) cell lysate, 10 µg

    Lane 2: Human lymph node cell lysate, 10 µg

    Secondary (all lanes): HRP labelled Goat anti Rabbit IgG at 1/2000 dilution.

    Predicted MW: 29 kDa.

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-CD27 antibody [EPR8569] (ab131254) purified at 1/5000 dilution.

    Lane 1: NAMALWA (human Burkitt's lymphoma cell line) cell lysate, 20 µg

    Secondary (all lanes): Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution.

    Predicted MW: 29 kDa.

    Observed MW: 55 kDa.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-CD27 antibody [EPR8569] (ab131254) purified, at 1/1000 dilution.

    Lane 1: Human lymph node tissue lysate, 20 µg

    Lane 2: Human fetal spleen tissue lysate, 20 µg

    Secondary (all lanes): Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution.

    Predicted MW: 29 kDa.

    Observed MW: 55 kDa.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)
    Western blot - Stimulatory immune checkpoint panel 2 - human WB (ab278177)

    All lanes: Anti-CD28 antibody [EPR22076] (ab243228) at 1/1000 dilution.

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate, 20 µg

    Lane 2: EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate, 20 µg

    Secondary (all lanes): ab131366 at 1/1000 dilution.

    Predicted MW: 25 kDa.

    Observed MW: The target band ranging between 37kDa-48kDa is consistent with what has been described in the literature (PMID: 2825196). Minor band below 15kDa is likely to be an isoform.

    This blot was developed using a higher sensitivity ECL substrate.

    This primary antibody was incubated overnight at 4°C.

    Exposure time: 26 secs.

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