Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD8 alpha antibody [EP1150Y] (ab93278)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1150Y] to CD8 alpha
Suitable for: WB, IHC-P, ICC
Reacts with: Human

Product name

Anti-CD8 alpha antibody [EP1150Y]
See all CD8 alpha primary antibodies
Description

Rabbit monoclonal [EP1150Y] to CD8 alpha
Host species

Rabbit
Specificity

The immunogen sequence for this antibody has no significant homology with mouse and rat CD alpha and beta. This product showed specific staining in lymphocytes, although we observed background staining in some epithelial cells tested.

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human CD8 alpha aa 200 to the C-terminus (C terminal).
Database link: P01732
Positive control
- WB: THP-1 cell lysate. IHC-P: Human tonsil tissue. ICC: MOLT-4 cells
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1150Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] (ab93278)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD8 alpha with purified ab93278 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry - Anti-CD8 alpha antibody [EP1150Y] (ab93278)

Immunocytochemistry analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) labeling CD8 alpha with purified ab93278 at 1/250 dilution (9 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.21 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Western blot - Anti-CD8 alpha antibody [EP1150Y] (ab93278)

Anti-CD8 alpha antibody [EP1150Y] (ab93278) at 1/1000 dilution (unpurified) + THP-1 cell lysate at 10 µg

Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] (ab93278)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil labelling CD8 alpha with unpurified ab93278 at a dilution of 1/250.
Anti-CD8 alpha antibody [EP1150Y] (ab93278)

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