Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
Key features and details
- Rabbit polyclonal to Cytochrome b245 Light Chain/p22-phox
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome b245 Light Chain/p22-phox antibody
See all Cytochrome b245 Light Chain/p22-phox primary antibodies -
Description
Rabbit polyclonal to Cytochrome b245 Light Chain/p22-phox -
Host species
Rabbit -
Specificity
Please note, previous lots of this product were guaranteed in ICC/IF. We are having trouble reproducing new lots suitable for ICC/IF. Therefore this application has been removed and it no longer guaranteed. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Cytochrome b245 Light Chain/p22-phox aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab86646) -
Positive control
- This antibody gave a positive signal in the following lysates: HepG2 Whole Cell, Mouse Pancreas Tissue, Mouse Spleen Tissue. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab75941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21 kDa). IHC-P Use a concentration of 1 µg/ml. Target
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Relevance
Cytochrome b245 Light Chain/p22phox is a critical component of the membrane-bound oxidase complex that generates superoxide. It complexes with NOX3 to form a functional NADPH oxidase. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 1535 Human
- Entrez Gene: 13057 Mouse
- Entrez Gene: 79129 Rat
- Omim: 608508 Human
- SwissProt: P13498 Human
- SwissProt: Q61462 Mouse
- SwissProt: Q62737 Rat
- Unigene: 513803 Human
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Alternative names
- CYBA antibody
- Cytochrome b 245 alpha polypeptide antibody
- Cytochrome b 245 light chain antibody
see all
Images
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Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)All lanes : Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Additional bands at: 19 kDa (possible cleavage fragment), 40 kDa. We are unsure as to the identity of these extra bands.We hypothesize that the 21 kDa band represents the pro-form of Cytochrome b245 Light Chain/p22-phox, and the 19 kDa band represents the mature form.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)Geng X et al., Int J Endocrinol 2016, Fig 3., doi: 10.1155/2016/2395634 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemical analyses of p22phox using ab75941 (1/200) in normal adrenocortical tissue (b-c) and adrenal aldosterone-producing adenoma, (e-f) p22phox presents a gradient of expression in the entire adrenal cortex (100x), and its localization was restricted to the cell membrane (400x). Sections were incubated with primary antibody at 4°C overnight and with a HRP-conjugated secondary antibody for 30 minutes at 37°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)IHC image of Cytochrome b245 Light Chain/p22-phox staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab75941, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
References (12)
ab75941 has been referenced in 12 publications.
- Yang X et al. Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation. Redox Biol 30:101432 (2020). PubMed: 31986467
- Feillet-Coudray C et al. Long-Term Measures of Dyslipidemia, Inflammation, and Oxidative Stress in Rats Fed a High-Fat/High-Fructose Diet. Lipids 54:81-97 (2019). PubMed: 30767221
- Sandoval R et al. TNF-a Increases Production of Reactive Oxygen Species through Cdk5 Activation in Nociceptive Neurons. Front Physiol 9:65 (2018). PubMed: 29467671
- Liang X et al. Reactive oxygen species mediated oxidative stress links diabetes and atrial fibrillation. Mol Med Rep 17:4933-4940 (2018). PubMed: 29393403
- Habtetsion T et al. Alteration of Tumor Metabolism by CD4+ T Cells Leads to TNF-a-Dependent Intensification of Oxidative Stress and Tumor Cell Death. Cell Metab 28:228-242.e6 (2018). PubMed: 29887396
Images
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Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)All lanes : Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Additional bands at: 19 kDa (possible cleavage fragment), 40 kDa. We are unsure as to the identity of these extra bands.We hypothesize that the 21 kDa band represents the pro-form of Cytochrome b245 Light Chain/p22-phox, and the 19 kDa band represents the mature form.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941) Geng X et al., Int J Endocrinol 2016, Fig 3., doi: 10.1155/2016/2395634 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Immunohistochemical analyses of p22phox using ab75941 (1/200) in normal adrenocortical tissue (b-c) and adrenal aldosterone-producing adenoma, (e-f) p22phox presents a gradient of expression in the entire adrenal cortex (100x), and its localization was restricted to the cell membrane (400x). Sections were incubated with primary antibody at 4°C overnight and with a HRP-conjugated secondary antibody for 30 minutes at 37°C.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941)
IHC image of Cytochrome b245 Light Chain/p22-phox staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab75941, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
