Anti-CD8 alpha antibody [BLR044F] (ab243900)
![Anti-CD8 alpha antibody [BLR044F] (ab243900)](https://www.abcam.com/ps/products/243/ab243900/Images/ab243900-334393-anti-cd8-antibody-blr044f-western-blot.jpg "Anti-CD8 alpha antibody [BLR044F] (ab243900)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR044F] to CD8 alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD8 alpha antibody [BLR044F]
See all CD8 alpha primary antibodies -
Description
Rabbit monoclonal [BLR044F] to CD8 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human CD8 alpha aa 185-235 (C terminal). The exact sequence is proprietary. NP_001759.3 and Gene ID 925.
Database link: P01732 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR044F -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD8 alpha antibody [BLR044F] (ab243900)
Western blot analysis using ab243900 at 1/1000 dilution.
Lane 1: CD4+ T cell whole cell lysate (50 µg).
Lane 2: CD8+ T cell whole cell lysate (50 µg).
Lane 3: Jurkat whole cell lysate (50 µg).
Lane 4: MOLT-4 whole cell lysate (50 µg).
Lane 5: RPMI 8226 whole cell lysate (50 µg).
Lane 6: MJ whole cell lysate (50 µg).
Lane 7: C5/MJ whole cell lysate (50 µg).
Lane 8: KG-1 whole cell lysate (50 µg).
Lane 9: SR whole cell lysate (50 µg).
Lane 10: K562 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] (ab243900)](https://www.abcam.com/ps/products/243/ab243900/Images/ab243900-334392-anti-cd8-antibody-blr044f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] (ab243900)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab243900 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining. -
![Anti-CD8 alpha antibody [BLR044F] (ab243900)](https://www.abcam.com/ps/products/243/ab243900/Images/ab243900-3-benefits-of-recombinant-antibodies.png)
Anti-CD8 alpha antibody [BLR044F] (ab243900)
