Anti-PARP1 antibody (ab218132)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PARP1 antibody
See all PARP1 primary antibodies -
Description
Rabbit polyclonal to PARP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PARP1 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P09874 -
Positive control
- Rat and mouse brain tissues. Rat brain lysates; HeLa and Raji cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab218132)
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PARP1 using ab218132 at a 1/200 dilution, followed by conjugation to the secondary antibody goat anti-rabbit IgG, FITC-conjugated at a 1/200 dilution and DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab218132)Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse brain tissue, labeling PARP1 using ab218132 at a 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-PARP1 antibody (ab218132)All lanes : Anti-PARP1 antibody (ab218132) at 1/200 dilution
Lane 1 : Human colon carcinoma lysates
Lane 2 : rat brain lysates
Secondary
All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 112 kDa why is the actual band size different from the predicted?
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Western blot - Anti-PARP1 antibody (ab218132)Anti-PARP1 antibody (ab218132) at 1/300 dilution + HeLa lysates
Secondary
goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
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Western blot - Anti-PARP1 antibody (ab218132)Anti-PARP1 antibody (ab218132) at 1/300 dilution + Human Raji lysates
Secondary
goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
