Anti-SNAP25 antibody (ab31281)
Key features and details
- Goat polyclonal to SNAP25
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SNAP25 antibody
See all SNAP25 primary antibodies -
Description
Goat polyclonal to SNAP25 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1 -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog
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Immunogen
Synthetic peptide corresponding to Human SNAP25 aa 193-206 (C terminal).
Sequence:C-DEANQRATKMLGSG
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAP25 antibody (ab31281)All lanes : Anti-SNAP25 antibody (ab31281) at 0.03 µg/ml
Lane 1 : Human brain (cerebellum) tissue lysate in RIPA buffer
Lane 2 : Mouse brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDaIncubated with the primary antibody for 1 hour.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab31281)ab31281 (1µg/ml) staining of SNAP25 in paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab31281)ab31281 negative control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab31281)ab31281 at 2.5µg/ml staining SNAP25 in human cerebral cortex tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
