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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs

Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)

Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3275] to SNAP25 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-SNAP25 antibody [EPR3275] - BSA and Azide free
    See all SNAP25 primary antibodies
  • Description

    Rabbit monoclonal [EPR3275] to SNAP25 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab171355 is the carrier-free version of ab109105. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab171355 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3275
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)

    This ICC data was generated using the same anti-SNAP25 antibody clone, EPR3275, in a different buffer formulation (cat# ab109105).

    Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100.

  • Flow Cytometry - Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)
    Flow Cytometry - Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)

    This Flow Cyt data was generated using the same anti-SNAP25 antibody clone, EPR3275, in a different buffer formulation (cat# ab109105).

    Flow cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling SNAP25 (red) with ab109105 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)
    Anti-SNAP25 antibody [EPR3275] - BSA and Azide free (ab171355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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