SNAP25 overexpression 293T lysate (whole cell) (ab94226)
Overview
-
Product name
SNAP25 overexpression 293T lysate (whole cell)
See all SNAP25 lysates -
General notes
ab94226 is a 293T cell transfected lysate in which Human SNAP25 has been transiently over-expressed using a pCMV-SNAP25 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes.
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
-
Background
Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Similarity: Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains. PTM: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
Images
-
SDS-PAGE - SNAP25 overexpression 293T lysate (whole cell) (ab94226)ab94226 at 15μg/lane on an SDS-PAGE gel
-
Western blot - SNAP25 overexpression 293T lysate (whole cell) (ab94226)All lanes : Anti-SNAP25 antibody (ab66066) at 1/500 dilution
Lane 1 :SNAP25 overexpression 293T lysate (whole cell) (ab94226)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
