Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SNAP25 antibody [EPR3275] (ab109105)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3275] to SNAP25
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-SNAP25 antibody [EPR3275]
See all SNAP25 primary antibodies
Description

Rabbit monoclonal [EPR3275] to SNAP25
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain tissue lysate and SH SY5Y, and Neuro 2a cell lysates. ICC/IF: SH SY5Y cells. Flow Cyt: SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3275
Isotype

IgG
Research areas

Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)

All lanes : Anti-SNAP25 antibody [EPR3275] (ab109105) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : SH SY5Y lysate
Lane 3 : Neuro 2a lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)

Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100.
Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)

Flow cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling SNAP25 (red) with ab109105 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Anti-SNAP25 antibody [EPR3275] (ab109105)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com