Anti-SNAP25 antibody (ab228880)
Key features and details
- Rabbit polyclonal to SNAP25
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SNAP25 antibody
See all SNAP25 primary antibodies -
Description
Rabbit polyclonal to SNAP25 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-Fr RatIHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Recombinant fragment within Human SNAP25 (internal sequence). The exact sequence is proprietary.
Database link: P60880 -
Positive control
- WB: Rat brain lysate; Mouse brain lysate; HeLa whole cell lysate. IHC-P: Mouse fore brain tiisue; Rat hind brain tissue; SW480 xenograft tissue. IHC-Fr: E13.5 rat brain tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAP25 antibody (ab228880)Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + Rat brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Western blot - Anti-SNAP25 antibody (ab228880)Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Western blot - Anti-SNAP25 antibody (ab228880)Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab228880)Paraffin-embedded mouse fore brain tissue stained for SNAP25 using ab228880 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab228880)Paraffin-embedded rat hind brain tissue stained for SNAP25 using ab228880 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (ab228880)Paraffin-embedded SW480 (human) xenograft tissue stained for SNAP25 using ab228880 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-SNAP25 antibody (ab228880)Frozen sectioned E13.5 rat brain tissue stained for SNAP25 using ab228880 at 1/250 dilution in immunohistochemical analysis (green).
The nuclear counterstain is DAPI (blue). Beta Tubulin 3, a mature neuron marker, is detected with a beta Tubulin 3 antibody at 1/500 dilution (red).
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Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody (ab228880)4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for SNAP25 (green) using ab228880 at 1/500 dilution in ICC/IF.
Nuclear counterstain: DAPI (blue). Beta Tubulin 3, a mature neuron marker, is detected with a beta Tubulin 3 antibody at 1/500 dilution (red).
