Antibodies, Proteins, Kits and Reagents for Life Science

Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1768Y] to GOSR1/GS28 - BSA and Azide free
Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free
See all GOSR1/GS28 primary antibodies
Description

Rabbit monoclonal [EP1768Y] to GOSR1/GS28 - BSA and Azide free
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested applications

Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human colon carcinoma tissue.
General notes

ab232339 is the carrier-free version of ab53288 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab232339 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as GOSR1
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1768Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified ab53288 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53288).
Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53288).
Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53288).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53288).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53288).
Anti-GOSR1/GS28 antibody [EP1768Y] - BSA and Azide free (ab232339)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com