Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1768Y] to GOSR1/GS28
Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-GOSR1/GS28 antibody [EP1768Y]
See all GOSR1/GS28 primary antibodies
Description

Rabbit monoclonal [EP1768Y] to GOSR1/GS28
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human GOSR1/GS28 (N terminal). The exact sequence is proprietary.
Positive control
- SKBR-3 cell lysate 3T3 cell lysate HeLa cells Human stomach carcinoma
General notes
This product was previously labelled as GOSR1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1768Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified ab53288 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

All lanes : Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288) at 1/1000 dilution (purified)

Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 6 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 7 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

All lanes : Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288) at 1/500 dilution (unpurified)

Lane 1 : SKBR-3 cell lysate
Lane 2 : 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution
Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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