Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SEC22B antibody [EPR12335] (ab181076)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12335] to SEC22B
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-SEC22B antibody [EPR12335]
See all SEC22B primary antibodies
Description

Rabbit monoclonal [EPR12335] to SEC22B
Host species

Rabbit
Specificity

The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain, Mouse spleen, Rat spleen, SH-SY5Y, MCF7 and HeLa lysates. IHC-P: human breast, ovarian carcinoma and mouse liver tissues. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y cells. IP: HeLa cells.
General notes
This product was previously labelled as SEC22L1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12335
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-SEC22B antibody [EPR12335] (ab181076)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SEC22B with purified ab181076 at 1/250 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-SEC22B antibody [EPR12335] (ab181076)

All lanes : Anti-SEC22B antibody [EPR12335] (ab181076) at 1/2000 dilution (Purified)

Lane 1 : Mouse brain lysates at 20 µg
Lane 2 : Mouse spleen lysates at 20 µg
Lane 3 : Rat spleen lysates

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 28 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC22B antibody [EPR12335] (ab181076)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling SEC22B with purified ab181076 at 1/250 dilution (2.19 µg/ml). Heat mediated antigen retrieval using Bond^TMEpitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-SEC22B antibody [EPR12335] (ab181076)

Anti-SEC22B antibody [EPR12335] (ab181076) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Western blot - Anti-SEC22B antibody [EPR12335] (ab181076)

Anti-SEC22B antibody [EPR12335] (ab181076) at 1/1000 dilution (Purified) + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-SEC22B antibody [EPR12335] (ab181076)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling SEC22B with purified ab181076 at 1/50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (ab181076)

ab181076 (purified ) at 1/30 dilution (2ug) immunoprecipitating SEC22B in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab181076 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181076 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC22B antibody [EPR12335] (ab181076)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SEC22B with purified ab181076 at 1/250 dilution (2.19 µg/ml). Heat mediated antigen retrieval using Bond^TM Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-SEC22B antibody [EPR12335] (ab181076)

Anti-SEC22B antibody [EPR12335] (ab181076) at 1/2000 dilution ((unpurified)) + SH-SY5Y cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SEC22B antibody [EPR12335] (ab181076)

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling SEC22B with unpurified ab181076 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunoprecipitation - Anti-SEC22B antibody [EPR12335] (ab181076)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using unpurified ab181076 at 1/30 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Flow Cytometry - Anti-SEC22B antibody [EPR12335] (ab181076)

Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling SEC22B with unpurified ab181076 at 1/50 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC22B antibody [EPR12335] (ab181076)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SEC22B with unpurified ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC22B antibody [EPR12335] (ab181076)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SEC22B with unpurified ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-SEC22B antibody [EPR12335] (ab181076)

Anti-SEC22B antibody [EPR12335] (ab181076) at 1/10000 dilution ((unpurified)) + MCF7 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa
Anti-SEC22B antibody [EPR12335] (ab181076)

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