Anti-Synapsin I (phospho S9) antibody (ab254033)
Key features and details
- Rabbit polyclonal to Synapsin I (phospho S9)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Synapsin I (phospho S9) antibody
See all Synapsin I primary antibodies -
Description
Rabbit polyclonal to Synapsin I (phospho S9) -
Host species
Rabbit -
Specificity
This antibody is specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser . Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser on synapsin I. Immunolabeling is completely eliminated by treatment with λ- Ptase.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human Synapsin I (phospho S9). The exact sequence is proprietary.
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Positive control
- ICC/IF: Cultured mouse caudate neurons. WB: Rat cortical lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: HEPES, 0.87% Sodium chloride, 50% Glycerol, 10% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Synapsin I (phospho S9) antibody (ab254033)All lanes : Anti-Synapsin I (phospho S9) antibody (ab254033) at 1/1000 dilution
Lane 1 : Un-treated rat cortical lysate
Lane 2 : Rat cortical lysate, treated with lambda phosphatase, 1200 units for 30 mins
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Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I (phospho S9) antibody (ab254033)Cultured mouse caudate neurons labeling Synapsin I (phospho S9) using ab254033 at a 1/500 dilution in ICC/IF.