Protein Carbonyl Content Assay Kit (Fluorometric) (ab235631)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum
- Sensitivity: 1 mg/ml
Overview
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Product name
Protein Carbonyl Content Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate -
Assay type
Quantitative -
Sensitivity
1 mg/ml -
Species reactivity
Reacts with: Human -
Product overview
Protein Carbonyl Content Assay Kit (Fluorometric) (ab235631) uses Fluoroscein-5-Thiosemicarbazide (FTC), a fluorescent probe which covalently reacts with oxidized residues (i.e. Cysteine, Lysine, Arginine, Histidine and Aspartic Acid) on proteins.
Protein carbonyl content is determined by the generation of a stable fluorometric signal (Ex/Em 485/535 nm), which is compared to the protein concentration determined in a BCA Assay to quantitate nmoles of carbonyl/mg protein.
The fluorometric protein carbonyl content assay kit is simple, requires no harsh chemicals, can quantitate carbonyls in serum or plasma and produces more reliable and reproducible results than the comparable colorimetric assays. It can detect carbonyl groups in samples with protein concentrations as low as 1 mg/mL.
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Notes
Related products
Other Protein Carbonyl Content assays include:
- Protein Carbonyl Content Assay (DNPH) ab126287 (most popular protein carbonyl content assay)
- Protein Carbonyl Content ELISA (DNPH) ab238536
- Protein Carbonyl Content Western Blot Assay ab178020Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Protein Carbonyl Assay Buffer NM 1 x 20ml 100% TCA Solution NM/Brown 1 x 20ml 10% Streptozotocin Solution Blue 1 x 1ml 6 M Guanidine Solution WM 1 x 20ml Sample Dilution Buffer Red/NM 1 x 50ml FTC (10 mM in DMSO) Orange 1 x 200µl Positive Control Protein (10 mg/ml) Violet 1 x 250µl
Images
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FTC Standard Curve.
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Evaluation of carbonyl content in human serum.50 µL of human serum from patients with Alzheimer’s Disease (AD) or healthy individuals were assayed following kit protocols.
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Comparison of AGE-BSA with untreated BSA illustrating the 7-fold increase of carbonyls on AGEs as compared to BSA.
