Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant dog GM-CSF protein (ab200292)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant dog GM-CSF protein
    See all GM-CSF proteins and peptides

  • Biological activity

    The ED50 determined by a cell proliferation assay using Human TF-1 cells is less than 4 ng/mL, corresponding to a specific activity of >2.5 x 105 IU/mg.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P48749

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Dog

    • Sequence

      APTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPE GPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQN NFKSFKENLKDFLFNIPFDCWKPVKK

    • Predicted molecular weight

      14 kDa

    • Amino acids

      18 to 144

    • Additional sequence information

      This product is for the mature full length protein. The signal peptide is not included. A single non-glycosylated polypeptide chain.

    • Specifications

    Our Abpromise guarantee covers the use of ab200292 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      HPLC

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store under desiccating conditions.

      pH: 7.40
      Constituent: 100% PBS

      Lyophilized from a 0.2 µM filtered solution.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

    General Info

    • Alternative names

      • Colony stimulating factor
      • Colony Stimulating Factor 2
      • Colony stimulating factor 2 (granulocyte-macrophage)
      • Colony-stimulating factor
      • CSF
      • CSF 2
      • CSF2
      • CSF2_HUMAN
      • GM-CSF
      • GMCSF
      • Granulocyte Macrophage Colony Stimulating Factor
      • Granulocyte-macrophage colony-stimulating factor
      • MGC131935
      • MGC138897
      • MGI1GM
      • Molgramostin
      • Pluripoietin-a
      • Sargramostim
      see all

    • Function

      Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

    • Sequence similarities

      Belongs to the GM-CSF family.

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab200292? Please let us know so that we can cite the reference in this datasheet.

ab200292 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Colony stimulating factor
  • Colony Stimulating Factor 2
  • Colony stimulating factor 2 (granulocyte-macrophage)

Images

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