Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
Key features and details
- Mouse monoclonal [8C3-D12-H10] to RPA70
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-RPA70 antibody [8C3-D12-H10]
See all RPA70 primary antibodies -
Description
Mouse monoclonal [8C3-D12-H10] to RPA70 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB RatHumanAfrican green monkey -
Immunogen
Recombinant fragment corresponding to Human RPA70. Purified recombinant human RPA70 protein fragments expressed in E.coli
Database link: P27694 -
Positive control
- COS7, HT1080, A431, MCF7, K562, HeLa, 293T, 3T3 and C6 cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
8C3-D12-H10 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution
Lane 1 : C6 Cell lysate
Lane 2 : COS7 Cell lysate
Lane 3 : K562 Cell lysate
Lane 4 : HeLa Cell lysate
Predicted band size: 68 kDa
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Immunocytochemistry/Immunofluorscence analysis of HeLa cells fixed with paraformaldehyde, labeling RPA70 with ab176467 at 1/100. Left - RPA70, Middle - nuclear staining, Right - Merge. This image was produced with antibody produced in ascites.
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : C6 cell lysate
Predicted band size: 68 kDaThis image was produced with antibody produced in ascites.
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
Lane 1 : COS7 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : HeLa cell lysate
Predicted band size: 68 kDaThis image was produced with antibody produced in ascites.
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Immunoprecipitation analysis of HeLa cell lysate labeling RPA70 with ab176467. This image was produced with antibody produced in ascites.