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Anti-RPA70 antibody [8C3-D12-H10] (ab176467)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8C3-D12-H10] to RPA70
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-RPA70 antibody [8C3-D12-H10]
    See all RPA70 primary antibodies
  • Description

    Mouse monoclonal [8C3-D12-H10] to RPA70
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    African green monkey
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human RPA70. Purified recombinant human RPA70 protein fragments expressed in E.coli
    Database link: P27694

  • Positive control

    • COS7, HT1080, A431, MCF7, K562, HeLa, 293T, 3T3 and C6 cell lysate
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Concentrated Culture Supernatant
  • Clonality

    Monoclonal
  • Clone number

    8C3-D12-H10
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Images

  • Western blot - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    Western blot - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution

    Lane 1 : C6 Cell lysate
    Lane 2 : COS7 Cell lysate
    Lane 3 : K562 Cell lysate
    Lane 4 : HeLa Cell lysate

    Predicted band size: 68 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    Immunocytochemistry/ Immunofluorescence - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)

    Immunocytochemistry/Immunofluorscence analysis of HeLa cells fixed with paraformaldehyde, labeling RPA70 with ab176467 at 1/100. Left - RPA70, Middle - nuclear staining, Right - Merge. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    Western blot - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : C6 cell lysate

    Predicted band size: 68 kDa



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    Western blot - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467)

    Lane 1 : COS7 cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : MCF7 cell lysate
    Lane 5 : K562 cell lysate
    Lane 6 : HeLa cell lysate

    Predicted band size: 68 kDa



    This image was produced with antibody produced in ascites. 

  • Immunoprecipitation - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
    Immunoprecipitation - Anti-RPA70 antibody [8C3-D12-H10] (ab176467)

    Immunoprecipitation analysis of HeLa cell lysate labeling RPA70 with ab176467. This image was produced with antibody produced in ascites. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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