Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19995] to Endostatin/COL18A1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free
    See all Endostatin/COL18A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19995] to Endostatin/COL18A1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251463 is the carrier-free version of ab207162 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251463 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Endostatin

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 178 kDa


    Exposure time: 3 minutes


    This data was developed using ab207162, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Negative control: U937 (PMID: 15451191).

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : Human seminoma lysate
    Lane 2 : Human testis lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 178 kDa



    This data was developed using ab207162, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 3: 1 minute; Lanes 2, 4-6: 3 minutes.

    The MW observed is consistent with the literature (PMID: 10716919; 15451191; 9687493; 11581192; 15545970; 21555590).

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) supernatant

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 178 kDa


    Exposure time: 3 minutes


    This data was developed using ab207162, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Endostatin and NC1 fragments from proteolytic cleavage were secreted into culture supernatant as described by literature (PMID: 1590618).

  • Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)
    Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (ab251463)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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