Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Endostatin/COL18A1 antibody [EPR22479-279] - BSA and Azide free (ab256508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22479-279] to Endostatin/COL18A1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Endostatin/COL18A1 antibody [EPR22479-279] - BSA and Azide free
    See all Endostatin/COL18A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22479-279] to Endostatin/COL18A1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta, skin and lung tissue lysates.

  • General notes

    Ab256508 is the carrier-free version of ab255601. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256508 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR22479-279] - BSA and Azide free (ab256508)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR22479-279] - BSA and Azide free (ab256508)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : Mouse placenta tissue lysate
    Lane 3 : Mouse skin tissue lysate
    Lane 4 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 182 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11581192, 16465514, 10506577).

    Negative control: RAW 264.7 (PMID: 27034233).

    This antibody reacts with an unidentifiable protein around 75 kDa in lane 1.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255601).

  • Anti-Endostatin/COL18A1 antibody [EPR22479-279] - BSA and Azide free (ab256508)
    Anti-Endostatin/COL18A1 antibody [EPR22479-279] - BSA and Azide free (ab256508)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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