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Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19995] to Endostatin/COL18A1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Endostatin/COL18A1 antibody [EPR19995]
    See all Endostatin/COL18A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19995] to Endostatin/COL18A1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta, seminoma, testis, fetal liver, fetal heart, fetal kidney and fetal spleen lysates; HepG2 whole cell lysate and supernatant.
  • General notes

     This product was previously labelled as Endostatin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19995
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors

Images

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 178 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: U937 (PMID: 15451191).

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : Human seminoma lysate
    Lane 2 : Human testis lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 178 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3: 1 minute; Lane 2/4-6: 3 minutes.

    The MW observed is consistent with the literature (PMID:10716919; PMID: 15451191; PMID: 9687493; PMID: 11581192; PMID: 15545970; PMID: 21555590).

  • Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) supernatant

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 178 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Endostatin and NC1 fragments from proteolytic cleavage were secreted into culture supernatant as described by literature (PMID:1590618).

  • Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
    Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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