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Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812]
    See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, Mouse heart, Rat brain and Rat heart lysates; 293T cells; HeLa cells.

  • General notes

     

     

     This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)
    Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)
    All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)
    Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)
    All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)
    Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)

    2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract (Input) 10 µg. Lane 2: ab197025 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)
    Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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