Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353)
Key features and details
- Rabbit polyclonal to 2 Hydroxy phytanoyl CoA lyase/HACL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody
See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies -
Description
Rabbit polyclonal to 2 Hydroxy phytanoyl CoA lyase/HACL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human 2 Hydroxy phytanoyl CoA lyase/HACL1 aa 528-578. The exact sequence is proprietary.
Database link: Q9UJ83 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, NIH/3T3 and TCMK-1 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to 2 Hydroxy phytanoyl CoA lyase/HACL1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353)All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 3 minutesPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody (ab241353)2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241353 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.Lane 1: ab241353 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Chemiluminescence detection: 30 seconds.
