Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DTX2 antibody [EPR10916] (ab170907)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10916] to DTX2
  • Suitable for: Flow Cyt, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DTX2 antibody [EPR10916]
    See all DTX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10916] to DTX2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DTX2 aa 500-600. The exact sequence is proprietary.
    Database link: Q86UW9

  • Positive control

    • HepG2 and MDA-MB-435 cell lysates, Human fetal heart lysate, Human cervical carcinoma and thyroid gland carcinoma tissues, HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10916

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)
    Western blot - Anti-DTX2 antibody [EPR10916] (ab170907)
    All lanes : Anti-DTX2 antibody [EPR10916] (ab170907) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MDA-MB-435 cell lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 67 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTX2 antibody [EPR10916] (ab170907)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, labeling DTX2 using ab170907 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)
    Flow Cytometry - Anti-DTX2 antibody [EPR10916] (ab170907)

    Flow cytometry analysis of permeabilized HepG2 cells labeling DTX2 (red), using ab170907 at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green)

  • Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)
    Immunoprecipitation - Anti-DTX2 antibody [EPR10916] (ab170907)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate showing DTX2, using ab170907 at 1/10 dilution.

  • Anti-DTX2 antibody [EPR10916] (ab170907)
    Anti-DTX2 antibody [EPR10916] (ab170907)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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