Anti-Cip4 antibody [EPR1966(2)] (ab108277)
![Anti-Cip4 antibody [EPR1966(2)] (ab108277)](https://www.abcam.com/ps/products/108/ab108277/Images/ab108277-410131-anti-cip4-antibody-epr19662-western-blot-human-knockout-lysate.jpg "Anti-Cip4 antibody [EPR1966(2)] (ab108277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1966(2)] to Cip4
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Cip4 antibody [EPR1966(2)]
See all Cip4 primary antibodies -
Description
Rabbit monoclonal [EPR1966(2)] to Cip4 -
Host species
Rabbit -
Specificity
The antibody has been tested in our lab in mouse and rat species without satisfactory results. Therefore, we do not recommend this antibody in these species. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, JAR, HepG2, TF1 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1966(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cip4 antibody [EPR1966(2)] (ab108277)All lanes : Anti-Cip4 antibody [EPR1966(2)] (ab108277) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TRIP10 knockout HEK293T cell lysate
Lane 3 : JAR cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108277 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108277 Anti-Cip4 antibody [EPR1966(2)] was shown to specifically react with Cip4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266428 (knockout cell lysate ab258251) was used. Wild-type and Cip4 knockout samples were subjected to SDS-PAGE. ab108277 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cip4 antibody [EPR1966(2)] (ab108277)](https://www.abcam.com/ps/products/108/ab108277/Images/Cip4-Primary-antibodies-ab108277-2.jpg)
Western blot - Anti-Cip4 antibody [EPR1966(2)] (ab108277)All lanes : Anti-Cip4 antibody [EPR1966(2)] (ab108277) at 1/1000 dilution
Lane 1 : JAR lysate
Lane 2 : HepG2 lysate
Lane 3 : TF1 lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa
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![Anti-Cip4 antibody [EPR1966(2)] (ab108277)](https://www.abcam.com/ps/products/108/ab108277/Images/ab108277-4-benefits-of-recombinant-antibodies.png)
Anti-Cip4 antibody [EPR1966(2)] (ab108277)
