Anti-Cip4 antibody (ab72220)
Key features and details
- Rabbit polyclonal to Cip4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cip4 antibody
See all Cip4 primary antibodies -
Description
Rabbit polyclonal to Cip4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A region between residues 551 and 601 of human Cip4
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Positive control
- HeLa cytoplasmic or membrane lysate 293T cytoplasmic or membrane lysate Mouse NIH3T3 cytoplasmic or membrane lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72220 was affinity purified using an epitope specific to Cip4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cip4 antibody (ab72220)All lanes : Anti-Cip4 antibody (ab72220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Immunoprecipitation - Cip4 antibody (ab72220)Detection of Human Cip4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with an antibody recognising an upstream epitope in lane 1, ab72220 at 3 µg in lane 2 and control IgG in lane 3. Image shows immunoprecipitated Cip4 detected using post IP WB, loading 20% of IP and using ab72220 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
