Anti-DDX17 antibody (ab24601)
Key features and details
- Rabbit polyclonal to DDX17
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX17 antibody
See all DDX17 primary antibodies -
Description
Rabbit polyclonal to DDX17 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human DDX17.
Read Abcam's proprietary immunogen policy (Peptide available as ab31640.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatDogApplication Abreviews Notes WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa).This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data).
Notes WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa).This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data).
Target
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Function
RNA-dependent ATPase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10521 Human
- Entrez Gene: 67040 Mouse
- Entrez Gene: 315133 Rat
- Omim: 608469 Human
- SwissProt: Q92841 Human
- SwissProt: Q501J6 Mouse
- Unigene: 528305 Human
- Unigene: 725500 Human
see all -
Alternative names
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
see all
Images
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Western blot - Anti-DDX17 antibody (ab24601)All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : Wild-type HEK 293 whole cell lysate
Lane 2 : DDX17 knockout HEK 293 whole cell lysate
Lane 3 : Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 80,73 kDaLanes 1 - 4: Merged signal (red and green). Green - ab24601 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab24601 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. Ab24601 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-DDX17 antibody (ab24601)All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80,73 kDa
Observed band size: 73,80 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab24601 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab24601 has been referenced in 8 publications.
- Lambert MP et al. The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. Nucleic Acids Res 46:7686-7700 (2018). PubMed: 29931089
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Lin B et al. The protective role of p72 in doxorubicin-induced cardiomyocytes injury in vitro. Mol Med Rep 14:3376-80 (2016). PubMed: 27510756
- Le Sage V et al. Proteomic analysis of HIV-1 Gag interacting partners using proximity-dependent biotinylation. Virol J 12:138 (2015). PubMed: 26362536
- Samaan S et al. The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24275493
- Germann S et al. Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor. Oncogene : (2012). PubMed: 22266867
- Mallon S et al. Use of biotinylated plasmid DNA as a surrogate for HSV DNA to identify proteins that repress or activate viral gene expression. Proc Natl Acad Sci U S A 109:E3549-57 (2012). ICC/IF ; Human . PubMed: 23223531
- Suzuki HI et al. Modulation of microRNA processing by p53. Nature 460:529-33 (2009). Flow Cyt ; Human . PubMed: 19626115
Images
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Western blot - Anti-DDX17 antibody (ab24601)All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : Wild-type HEK 293 whole cell lysate
Lane 2 : DDX17 knockout HEK 293 whole cell lysate
Lane 3 : Hela whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 80,73 kDaLanes 1 - 4: Merged signal (red and green). Green - ab24601 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab24601 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. Ab24601 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-DDX17 antibody (ab24601)All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80,73 kDa
Observed band size: 73,80 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab24601 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
