Anti-DDX17 antibody [2248C2a] (ab71958)
![Anti-DDX17 antibody [2248C2a] (ab71958)](https://www.abcam.com/ps/products/71/ab71958/Images/ab71958-353454-antiddx17antibody2248c2awesternblotknockouthek293ddx17helahumanlysates.jpg "Anti-DDX17 antibody [2248C2a] (ab71958)")
Key features and details
- Mouse monoclonal [2248C2a] to DDX17
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-DDX17 antibody [2248C2a]
See all DDX17 primary antibodies -
Description
Mouse monoclonal [2248C2a] to DDX17 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Recombinant fragment of human DDX17
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Positive control
- Recombinant human DDX17. HEK-293 and HeLa cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab71958 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
2248C2a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)All lanes : Anti-DDX17 antibody [2248C2a] (ab71958) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : DDX17 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 72 kDaLanes 1 - 3: Merged signal (red and green). Green - ab71958 observed at 72, 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71958 was shown to recognize DDX17 in wild-type HEK-293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. Ab71958 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)](https://www.abcam.com/ps/products/71/ab71958/Images/ab71958-86996-anti-ddx17-antibody-2248c2a-western-blot.gif)
Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)Anti-DDX17 antibody [2248C2a] (ab71958) + immunising recombinant protein
Predicted band size: 72 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
