Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13807(B)] to DDX17 - C-terminal
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-DDX17 antibody [EPR13807(B)] - C-terminal
    See all DDX17 primary antibodies

  • Description

    Rabbit monoclonal [EPR13807(B)] to DDX17 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DDX17 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92841

  • Positive control

    • WB: HeLa, NIH/3T3, J774A.1, RAW264.7, C2C12, 293T, HeLa and MCF7 cell lysates; Mouse embryo lysate. IHC-P: Human breast tissue. ICC/IF: 293T and MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13807(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/5000 dilution

    Lane 1 : Wild-type HEK 293 whole cell lysate
    Lane 2 : DDX17 knockout HEK 293 whole cell lysate
    Lane 3 : Hela whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 72 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab180190 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180190 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab180190 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)

    ab180190 staining DDX17 antibody in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)

    Immunohistochemical analysis of paraffin embedded Human breast tissue labeling DDX17 with ab180190 at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Flow Cytometry - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)

    Flow Cytometrical analysis of permeabilized MCF7 cells labeling DDX17 with ab180190 at 1/10 (red) or a rabbit IgG negative (green).

  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution

    Lane 1 : HeLa (Human colorectal adenocarcinoma epithelial cell) cell lysate
    Lane 2 : Mouse embryo lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
    Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cell lysate
    Lane 5 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate
    Lane 6 : C2C12 (Mouse myoblasts myoblast) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 6 s

  • Western blot - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Western blot - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    All lanes : Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 72 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)

    Immunofluorescent analysis of 293T cells labeling DDX17 with ab180190 at 1/100.

  • Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)
    Anti-DDX17 antibody [EPR13807(B)] - C-terminal (ab180190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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