Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23221-54] to Eph receptor B4/HTK - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free
    See all Eph receptor B4/HTK primary antibodies

  • Description

    Rabbit monoclonal [EPR23221-54] to Eph receptor B4/HTK - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HT-29 and MCF7 lysates. IP: HT-29 and HUVEC whole cell lysates.

  • General notes

    ab269867 is the carrier-free version of ab254300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
    Western blot - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
    All lanes : Anti-Eph receptor B4/HTK antibody [EPR23221-54] (ab254300) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : EPHB4 knockout HEK293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HT-29 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 108 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab254300).

    Lanes 1-4: Merged signal (red and green). Green - ab254300 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab254300 Anti-Eph receptor B4/HTK antibody [EPR23221-54] was shown to specifically react with Eph receptor B4/HTK in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266733 (knockout cell lysate ab257429) was used. Wild-type and Eph receptor B4/HTK knockout samples were subjected to SDS-PAGE. ab254300 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
    Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

    Eph receptor B4 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab254300 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254300 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10ug

    Lane 2: ab254300 IP in HUVEC whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254300 in HUVEC whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    Cleaved intracellular domain (ICD) was observed at around 47kDa. (PMID: 24854540).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254300).

  • Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
    Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

    Eph receptor B4 was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab254300 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254300 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab254300 IP in HT-29 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254300 in HT-29 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    Cleaved intracellular domain (ICD) was observed at around 47kDa. (PMID: 24854540).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254300).

  • Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
    Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

© 2021 Astana Biomed Group LLP. All rights reserved.